FROM RECRUITMENT TO BIRTH

The BiSC cohort has received funding from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (785994 – AirNB project), from the Health Effects Institute (4959-RFA17-1/18-1 – FRONTIER project), from the European Union’s Horizon 2020 research and innovation programme-EU.3.1.2. (874583 – ATHLETE project) and H2020-EU.3.1.1. (GA964827 – AURORA project), from AXA Research Fund (MOOD-COVID project), from Agence nationale de sécurité sanitaire de l’alimentation, de l’environnement et du travail (ANSES) ( 2019/01/039 – HyPAXE project), from the AGAUR-Agència de Gestió d’Ajuts Universitaris de Recerca (2017 SGR 826 – Population Neuroscience group), from the Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP) (CB06/02/0041), from the Instituto de Salud Carlos III (ISCIII) and the European Regional Development Fund (ERDF) – Maternal and Child Health and Development Network (SAMID) (RD16/0022/0014 and RD16/0022/0015), and from the Instituto de Salud Carlos III (ISCIII) and the European Union Next Generation EU – Primary Care Interventions to Prevent Maternal and Child Chronic Diseases of Perinatal and Developmental Origin Network (RICORS-SAMID) (RD21/0012/0001 and RD21/0012/0003).

Specific for some datasets:

  • Genome-wide genotyping data was funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI20/01116 – ENTENTE project) and the Centro Nacional de Genotipado-CEGEN (PRB2-ISCIII).
  • Placental mitochondrial DNA and telomere length data was funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI17/01142 – AirPLASM project).
  • Placental DNA methylation was funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI20/00190 – ALMA project), and from the European Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL and Instituto de Salud Carlos III) (AC18/00006 – NutriPROGRAM project).
  • Placental small non-coding RNAs were funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI19/00417) and from the Fundació Academia de Ciències Mèdiques de Catalunya i Balears (Premi Ciències Biomèdiques 2019).
  • Maternal steroid hormones were funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI21/01269 – IGRO project).
  • Maternal blood polyunsaturated long chain fatty acids (PUFAs) and urine polyphenol determinations during pregnancy were funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI21/00266 – NUTINBRAIN project).
  • Maternal serum and urine and placental exposomics data was funded by the Ministerio de Ciencia e innovación (20213AT019).

For studies dealing with FRONTIER’s exposure-outcomes (air pollution, noise, and greenspace in association with foetal growth, placental function, and hair cortisol level), this text has to be added:

“Research described in this article was conducted under contract to the Health Effects Institute (HEI), an organization jointly funded by the United States Environmental Protection Agency (EPA) (Assistance Award No. R-82811201) and certain motor vehicle and engine manufacturers. The contents of this article do not necessarily reflect the views of HEI, or its sponsors, nor do they necessarily reflect the views and policies of the EPA or motor vehicle and engine manufacturers”.

FROM BIRTH TO 28 MONTHS

The BiSC cohort has received funding for the follow-up visits up to 18 months of age from the European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme (785994 – AirNB project), from the California Walnut Commission (976061), from the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI21/00266 –NUTINBRAIN), from the AGAUR-Agència de Gestió d’Ajuts Universitaris de Recerca (2021 SGR 01570 – Population Neuroscience group), and from the Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP – CB06/02/0041).

Specific for some datasets:

  • Gut microbiota data at age 6 and 18 months was funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI21/01278 – ALTER project). The salary of the bioinformatician of the metagenomics data was funded by an INVESTIGO contract (100046TC1).
  • Nanoplastic measurement in stool samples was funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI23/00615 – PLASTICINE project).

FOUR-YEARS VISIT

The BiSC cohort has received funding for the 4-years follow-up visit by La Marató de TV3 – Malalties mentals (Agreement 202216-30-31 – ENGENE project), from the California Walnut Commission (976061), from the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (PI21/00266 –NUTINBRAIN), from the AGAUR-Agència de Gestió d’Ajuts Universitaris de Recerca (2021 SGR 01570 – Population Neuroscience group), and from the Centro de Investigación Biomédica en Red de Epidemiología y Salud Pública (CIBERESP – CB06/02/0041). Technicians of the visit were funded by the following INVESTIGO grants: 100046TC15, 100046TC18, 100046TC10, 100046TC13, 100046TC28.

Specific for some datasets:

  • Cardiovascular risk assessment in BiSC mothers was funded by the Instituto de Salud Carlos III (ISCIII) and co-funded by European Union (ERDF) “A way to make Europe” (CARDIOMOM – PI22/00462).
  • Immune measurements in child blood was funded by the European Union’s Horizon 2020 research and innovation programme HE-HLTH-2023-ENVHLTH (101136566 – ENDOMIX project).

GENERAL FOR ISGLOBAL

We acknowledge support from the grant CEX2018-000806-S funded by MCIN/AEI/ 10.13039/501100011033, and support from the Generalitat de Catalunya through the CERCA Program.

GENERAL FOR REDCAP

Study data were collected and managed using REDCap electronic data capture tools hosted at ISGlobal (REF 1 and REF 2). REDCap (Research Electronic Data Capture) is a secure, web-based software platform designed to support data capture for research studies, providing 1) an intuitive interface for validated data capture; 2) audit trails for tracking data manipulation and export procedures; 3) automated export procedures for seamless data downloads to common statistical packages; and 4) procedures for data integration and interoperability with external sources.

REF 1. PA Harris, RTaylor, R Thielke, J Payne, N Gonzalez, JG. Conde. Research electronic data capture (REDCap) – A meta data-driven methodology and work flow process for providing translational research informatics support. JBiomedInform. 2009 Apr;42(2):377-81. 10.1016/j.jbi.2008.08.010

REF 2. PA Harris, R Taylor, BL Minor, V Elliott, M Fernandez, L O’Neal, L McLeod, G Delacqua, F Delacqua, J Kirby, S N Duda, REDCap Consortium. The REDCap consortium: Building an international community software partners. JBiomedInform. 2019 May9 10.1016/j.jbi.2019.103208

ETHICS

From pregnancy up to 18 months visits were approved by the Clinical Research Ethics Committee of the Parc de Salut Mar project (2018/8050/I), Medical Research Committee of the Fundació de Gestió Sanitària del Hospital de la Santa Creu i Sant Pau de Barcelona (EC/18/206/5272), and Ethics Committee of the Fundació Sant Joan de Déu (PIC-27-18). Visits at 24 months, 28 months and 48 months were also approved by the Clinical Research Ethics Committee of the Parc de Salut Mar project (2018/8050/I), as well as the ENDOMIX project (2023/11304/I). Before joining the cohort during their regular first trimester hospital visit, participants were informed by a BiSC midwife or nurse about the study’s details, duration, and their option to withdraw without penalty. If they agreed to take part, they signed consent forms permitting the collection of biological samples and genetic studies, receiving a copy for themselves. Parents or legal guardians of children also provided informed consent before any study procedures commenced. Specific consent forms for genetic studies were also provided to mothers and parents or legal guardians of children.